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验证数据展示

  • WB analysis using 85217-4-RR

    WB analysis using 85217-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skin using 85217-4-RR

    WB analysis of mouse skin using 85217-4-RR

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85217-4-RR

    Affinity and Kinetic Characterization of 85217-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85217-4-RR against Human Plakophilin 1 were performed. The affinity constant is 11.4 nM.

Plakophilin 1 Recombinant monoclonal antibody

Plakophilin 1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 85217-4-RR

产品说明书

CloneNo. 242834G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

PKP1, 242834G8, B6P, Plakophilin-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue, HEK-293 cells, A431 cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85217-4-RR targets Plakophilin 1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18153

Product name: Recombinant human PKP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 412-726 aa of BC114571

Sequence: NLSSADAGRQTMRNYSGLIDSLMAYVQNCVAASRCDDKSVENCMCVLHNLSYRLDAEVPTRYRQLEYNARNAYTEKSSTGCFSNKSDKMMNNNYDCPLPEEETNPKGSGWLYHSDAIRTYLNLMGKSKKDATLEACAGALQNLTASKGLMSSGMSQLIGLKEKGLPQIARLLQSGNSDVVRSGASLLSNMSRHPLLHRVMGNQVFPEVTRLLTSHTGNTSNSEDILSSACYTVRNLMASQPQLAKQYFSSSMLNNIINLCRSSASPKAAEAARLLLSDMWSSKELQGVLRQQGFDRNMLGTLAGANSLRNFTSRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
别名 PKP1, 242834G8, B6P, Plakophilin-1
计算分子量 747 aa, 83 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC114571
基因名称 PKP1
Gene ID (NCBI) 5317
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13835
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).

实验方案

Product Specific Protocols
WB protocol for Plakophilin 1 antibody 85217-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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