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验证数据展示

  • WB analysis of mouse spleen using 85010-1-RR

    WB analysis of mouse spleen using 85010-1-RR

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 85010-1-RR (LAIR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 85010-1-RR

    IHC staining of mouse spleen using 85010-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85010-1-RR (LAIR1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 85010-1-RR

    IHC staining of mouse spleen using 85010-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85010-1-RR (LAIR1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using 85010-1-RR

    IF Staining of RAW 264.7 using 85010-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using LAIR1 antibody (85010-1-RR, Clone: 242226B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85010-1-RR

    Affinity and Kinetic Characterization of 85010-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85010-1-RR against Mouse LAIR1 were performed. The affinity constant is below 1 pM.

LAIR1 Recombinant monoclonal antibody

LAIR1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85010-1-RR

产品说明书

CloneNo. 242226B10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, ELISA

别名

242226B10, CD305, LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1, mLAIR1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85010-1-RR targets LAIR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1879

Product name: Recombinant Mouse LAIR1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-141 aa of NM_001113474.1

Sequence: QEGSLPDITIFPNSSLMISQGTFVTVVCSYSDKHDLYNMVRLEKDGSTFMEKSTEPYKTEDEFEIGPVNETITGHYSCIYSKGITWSERSKTLELKVIKENVIQTPAPGPTSDTSWLKTY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leukocyte-associated Ig-like receptor 1
别名 242226B10, CD305, LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1, mLAIR1
计算分子量30kDa
观测分子量35 kDa, 45 kDa
GenBank蛋白编号NM_001113474.1
基因名称 Lair1
Gene ID (NCBI) 52855
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8BG84-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

实验方案

Product Specific Protocols
IF protocol for LAIR1 antibody 85010-1-RRDownload protocol
WB protocol for LAIR1 antibody 85010-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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