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Species Reactivity
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验证数据展示

  • WB analysis using 84966-2-RR (same clone as 84966-2-PBS)

    WB analysis using 84966-2-RR (same clone as 84966-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84966-2-RR (Eg5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 84966-2-RR (same clone as 84966-2-PBS)

    IHC staining of human lung cancer using 84966-2-RR (same clone as 84966-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84966-2-RR (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84966-2-RR (same clone as 84966-2-PBS)

    IF Staining of HepG2 using 84966-2-RR (same clone as 84966-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (84966-2-RR, Clone: 242611D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84966-2-RR (same clone as 84966-2-PBS)

    FC experiment of HeLa using 84966-2-RR (same clone as 84966-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Eg5 Recombinant antibody (84966-2-RR, Clone:242611D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84966-2-RR

    Affinity and Kinetic Characterization of 84966-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84966-2-RR against Human Eg5 were performed. The affinity constant is 18.3 nM.

Eg5 Recombinant antibody, PBS Only

Eg5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84966-2-PBS

产品说明书

CloneNo. 242611D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

KIF11, 242611D10, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84966-2-PBS targets Eg5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Eg5 fusion protein Ag20001 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member 11
别名 KIF11, 242611D10, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP
计算分子量 1056 aa, 119 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC136474
基因名称 Eg5
Gene ID (NCBI) 3832
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52732
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.