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验证数据展示

  • WB analysis of mouse thymus using 84878-5-RR

    WB analysis of mouse thymus using 84878-5-RR

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 84878-5-RR (TCB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84878-5-RR

    WB analysis using 84878-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84878-5-RR (TCB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 84878-5-RR

    IHC staining of mouse spleen using 84878-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84878-5-RR (TCB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse thymus using 84878-5-RR

    IF Staining of mouse thymus using 84878-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using TCB1 antibody (84878-5-RR, Clone: 241802B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TCB1 Recombinant antibody

TCB1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84878-5-RR

产品说明书

CloneNo. 241802B12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

241802B12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEL-4 cells, mouse thymus tissue, mouse brain tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue, mouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse thymus tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84878-5-RR targets TCB1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 TCB1 fusion protein Eg2437 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TCB1
别名 241802B12
计算分子量19 kDa
观测分子量38 kDa
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01852
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCB1 antibody 84878-5-RRDownload protocol
IHC protocol for TCB1 antibody 84878-5-RRDownload protocol
IF protocol for TCB1 antibody 84878-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols