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验证数据展示

  • IF Staining of MCF-7 using 84762-4-RR

    IF Staining of MCF-7 using 84762-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CACNB3 antibody (84762-4-RR, Clone: 242088E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of SKOV-3 using 84762-4-RR

    FC experiment of SKOV-3 using 84762-4-RR

    1x10^6 SKOV-3 cells were intracellularly stained with 0.25 ug CACNB3 Recombinant antibody (84762-4-RR, Clone:242088E4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84762-4-RR

    Affinity and Kinetic Characterization of 84762-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84762-4-RR against Human CACNB3 were performed. The affinity constant is 0.191 nM.

CACNB3 Recombinant monoclonal antibody

CACNB3 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84762-4-RR

产品说明书

CloneNo. 242088E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

242088E4, CAB3, CACNB 3, CACNLB3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inSKOV-3 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84762-4-RR targets CACNB3 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35651

Product name: Recombinant human CACNB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 351-484 aa of NM_000725.3

Sequence: EYLEVYWRATHHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQRSSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWPKDSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium channel, voltage-dependent, beta 3 subunit
别名 242088E4, CAB3, CACNB 3, CACNLB3
计算分子量55kDa，484aa
GenBank蛋白编号NM_000725.3
基因名称 CACNB3
Gene ID (NCBI) 784
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP54284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACNB3, also known as CACNLB3, is a voltage-gated Ca2+ channel subunit. It has 5 isoforms and may play a role in the regulation of transcription factors and calcium transport.

实验方案

Product Specific Protocols
IF protocol for CACNB3 antibody 84762-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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