• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84143-1-RR

    WB analysis using 84143-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84143-1-RR (CDH26 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-2 OS using 84143-1-RR

    FC experiment of U-2 OS using 84143-1-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CDH26 Recombinant antibody

CDH26 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84143-1-RR

产品说明书

CloneNo. 240934F9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Cadherin-like protein VR20, Cadherin-like protein 26, 240934F9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HEK-293 cells, T-47D cells
Positive FC (Intra) detected inU-2 OS, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84143-1-RR targets CDH26 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin-like 26
别名 Cadherin-like protein VR20, Cadherin-like protein 26, 240934F9
计算分子量 95 kDa
观测分子量18 kDa
GenBank蛋白编号NM_021810
基因名称 CDH26
Gene ID (NCBI) 60437
RRIDAB_3671705
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IXH8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for CDH26 antibody 84143-1-RRDownload protocol
FC protocol for CDH26 antibody 84143-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols