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验证数据展示

  • WB analysis using 84039-6-RR

    WB analysis using 84039-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84039-6-RR (PARP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 84039-6-RR

    IHC staining of mouse colon using 84039-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84039-6-RR (PARP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84039-6-RR

    Affinity and Kinetic Characterization of 84039-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84039-6-RR against Human PARP14 were performed. The affinity constant is 0.685 nM.

PARP14 Recombinant monoclonal antibody

PARP14 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84039-6-RR

产品说明书

CloneNo. 241078C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PARP 14, KIAA1268, BAL2, 241078C11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, U2OS cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84039-6-RR targets PARP14 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35007

Product name: Recombinant human PARP14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1501-1640 aa of NM_017554

Sequence: SRDVMQARDEIEAMIKRVRLAKEQESRADCISEFIEWQYNDNNTSHCFNKMTNLKLEDARREKKKTVDVKINHRHYTVNLNTYTATDTKGHSLSVQRLTKSKVDIPAHWSDMKQQNFCVVELLPSDPEYNTVASKFNQTC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 14
别名 PARP 14, KIAA1268, BAL2, 241078C11
计算分子量203kd
观测分子量200 kDa
GenBank蛋白编号NM_017554
基因名称 PARP14
Gene ID (NCBI) 54625
RRIDAB_3671606
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ460N5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion (PMID: 25753673). The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability (PMID: 37703374).

实验方案

Product Specific Protocols
IHC protocol for PARP14 antibody 84039-6-RRDownload protocol
WB protocol for PARP14 antibody 84039-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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