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验证数据展示

  • IHC staining of mouse spleen using 83941-7-RR

    IHC staining of mouse spleen using 83941-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83941-7-RR (LAG-3/CD223 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83941-7-RR

    IHC staining of mouse spleen using 83941-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83941-7-RR (LAG-3/CD223 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 83941-7-RR

    IF Staining of mouse spleen using 83941-7-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using LAG-3/CD223 antibody (83941-7-RR, Clone: 241007B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 83941-7-RR

    IF Staining of mouse spleen using 83941-7-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using LAG-3/CD223 antibody (83941-7-RR, Clone: 241007B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LAG-3/CD223 Recombinant monoclonal antibody

LAG-3/CD223 Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 83941-7-RR

产品说明书

CloneNo. 241007B1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF-P, ELISA

别名

CD223, LAG-3, LAG 3, Lag3, Ly66

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83941-7-RR targets LAG-3/CD223 in IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1220

Product name: Recombinant Mouse LAG-3/CD223 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 24-442 aa of NM_008479.2

Sequence: GPGKELPVVWAQEGAPVHLPCSLKSPNLDPNFLRRGGVIWQHQPDSGQPTPIPALDLHQGMPSPRQPAPGRYTVLSVAPGGLRSGRQPLHPHVQLEERGLQRGDFSLWLRPALRTDAGEYHATVRLPNRALSCSLRLRVGQASMIASPSGVLKLSDWVLLNCSFSRPDRPVSVHWFQGQNRVPVYNSPRHFLAETFLLLPQVSPLDSGTWGCVLTYRDGFNVSITYNLKVLGLEPVAPLTVYAAEGSRVELPCHLPPGVGTPSLLIAKWTPPGGGPELPVAGKSGNFTLHLEAVGLAQAGTYTCSIHLQGQQLNATVTLAVITVTPKSFGLPGSRGKLLCEVTPASGKERFVWRPLNNLSRSCPGPVLEIQEARLLAERWQCQLYEGQRLLGATVYAAESSSGAHSARRISGDLKGGHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte-activation gene 3
别名 CD223, LAG-3, LAG 3, Lag3, Ly66
计算分子量57 kDa
GenBank蛋白编号NM_008479.2
基因名称 CD223
Gene ID (NCBI) 16768
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ61790
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

实验方案

Product Specific Protocols
IF protocol for LAG-3/CD223 antibody 83941-7-RRDownload protocol
IHC protocol for LAG-3/CD223 antibody 83941-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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