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验证数据展示

  • WB analysis using 83853-2-RR (same clone as 83853-2-PBS)

    WB analysis using 83853-2-RR (same clone as 83853-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

    IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

    IHC staining of mouse spleen using 83853-2-RR (same clone as 83853-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83853-2-RR (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83853-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83853-2-RR

    Affinity and Kinetic Characterization of 83853-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83853-2-RR against Rat Lipocalin-2/Ngal were performed. The affinity constant is 0.195 nM.

Rat Lipocalin-2/NGAL Recombinant antibody, PBS Only

Lipocalin-2/NGAL Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83853-2-PBS

产品说明书

CloneNo. 240792C12

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Lcn2, Lipocalin2/NGAL, 240792C12, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83853-2-PBS targets Lipocalin-2/NGAL in WB, IHC, Cytometric bead array, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 Lipocalin-2/NGAL fusion protein Eg1022 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lipocalin 2
别名 Lcn2, Lipocalin2/NGAL, 240792C12, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein
计算分子量22kDa
观测分子量22-25 kDa
基因名称 Lipocalin-2/NGAL
Gene ID (NCBI) 170496
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30152
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/Ngal.