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验证数据展示

  • FC experiment of SH-SY5Y using 83660-1-RR

    FC experiment of SH-SY5Y using 83660-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • WB analysis using 83660-1-RR

    WB analysis using 83660-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83660-1-RR

    Affinity and Kinetic Characterization of 83660-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

SOX4 Recombinant antibody

SOX4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83660-1-RR

产品说明书

CloneNo. 240625E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Transcription factor SOX-4, EVI16, CSS10, 240625E3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A431 cells, MCF-7 cells, SGC-7901 cells
Positive FC (Intra) detected inSH-SY5Y cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83660-1-RR targets SOX4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SOX4 fusion protein Ag26583 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 4
别名 Transcription factor SOX-4, EVI16, CSS10, 240625E3
计算分子量 474 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC072668
基因名称 SOX4
Gene ID (NCBI) 6659
RRIDAB_3671267
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ06945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa.

实验方案

Product Specific Protocols
WB protocol for SOX4 antibody 83660-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols