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验证数据展示

  • WB analysis of HepG2 using 83536-1-RR

    WB analysis of HepG2 using 83536-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83536-1-RR (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83536-1-RR

    FC experiment of U2OS using 83536-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Fem1c Recombinant Antibody (83536-1-RR, Clone:240126G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83536-1-RR

    Affinity and Kinetic Characterization of 83536-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83536-1-RR against Human FEM1C were performed. The affinity constant is 0.896 nM.

FEM1C Recombinant monoclonal antibody

FEM1C Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83536-1-RR

产品说明书

CloneNo. 240126G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy FEM1C Ready-To-Use IHC Kit. FEM1C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83536-1-RR targets FEM1C in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5099

Product name: Recombinant human FEM1C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 315-617 aa of BC028369

Sequence: PDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFKRCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFMLQDRAKGLLGTTVTFDDLMGILCKSVLEIERAIKQTQCPADPLQLNKALSIILHLICLLEKVPCTLEQDHFKKQTIYRFLKLHPRGKNNFSPLHLAVDKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNNHPDIMNLLIKSGAHFDATNLHKQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGHIPEKLETFVSLHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fem-1 homolog c (C. elegans)
别名 FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM
计算分子量 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC028369
基因名称 FEM1C
Gene ID (NCBI) 56929
RRIDAB_3671160
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96JP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.

实验方案

Product Specific Protocols
WB protocol for FEM1C antibody 83536-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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