验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83536-1-RR targets FEM1C in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | FEM1C fusion protein Ag5099 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | fem-1 homolog c (C. elegans) |
| 别名 | FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM |
| 计算分子量 | 69 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC028369 |
| 基因名称 | FEM1C |
| Gene ID (NCBI) | 56929 |
| RRID | AB_3671160 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q96JP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FEM1C antibody 83536-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |