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验证数据展示

  • WB analysis using 83471-5-RR

    WB analysis using 83471-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83471-5-RR

    IF Staining of U2OS using 83471-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of U2OS using 83471-5-RR

    FC experiment of U2OS using 83471-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug IKZF4 Recombinant antibody (83471-5-RR, Clone:240357D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83471-5-RR

    Affinity and Kinetic Characterization of 83471-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83471-5-RR against Human IKZF4 were performed. The affinity constant is 21.3 nM.

IKZF4 Recombinant antibody

IKZF4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83471-5-RR

产品说明书

CloneNo. 240357D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

ZNFN1A4, Zinc finger protein Eos, KIAA1782, EOS, 240357D5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, K-562 cells, HepG2 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83471-5-RR targets IKZF4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 IKZF4 fusion protein Ag34767 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IKAROS family zinc finger 4 (Eos)
别名 ZNFN1A4, Zinc finger protein Eos, KIAA1782, EOS, 240357D5
观测分子量60 kDa
GenBank蛋白编号BC160169
基因名称 IKZF4
Gene ID (NCBI) 64375
RRIDAB_3671101
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H2S9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IKZF4 is a member of the Ikaros family transcription factors. The Ikaros family was originally designated to function in hematopoiesis, but it is only recently that studies have begun to show the relevance of this family in T helper cell differentiation. IKZF3 was preferentially expressed in TH17 cells and governs TH17 differentiation by silencing IL-2 expression. By investigating the dynamics of the TH17 regulatory network, IKZF4 was proposed by Yosef et al. as a composite in the negative regulatory module for TH17. (PMID: 24644282)

实验方案

Product Specific Protocols
WB protocol for IKZF4 antibody 83471-5-RRDownload protocol
IF protocol for IKZF4 antibody 83471-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols