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验证数据展示

  • WB analysis using 83398-1-RR

    WB analysis using 83398-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83398-1-RR (MOSPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 83398-1-RR

    WB analysis of HeLa using 83398-1-RR

    WB result of MOSPD2 antibody (83398-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells.

  • IHC staining of mouse brain using 83398-1-RR

    IHC staining of mouse brain using 83398-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MOSPD2 antibody included
  • IHC staining of mouse placenta using 83398-1-RR

    IHC staining of mouse placenta using 83398-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MOSPD2 antibody included
  • FC experiment of A431 using 83398-1-RR

    FC experiment of A431 using 83398-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MOSPD2 Recombinant antibody (83398-1-RR, Clone:240282C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83398-1-RR

    Affinity and Kinetic Characterization of 83398-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83398-1 -RR against Human MOSPD2 were performed. The affinity constant is 0.245 nM.

  • Featured Product
  • KD/KO验证

MOSPD2 Recombinant monoclonal antibody

MOSPD2 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83398-1-RR

产品说明书

CloneNo. 240282C11

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, FC (Intra), ELISA

别名

240282C11, Motile sperm domain-containing protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuH-7 cells, HeLa cells, Jurkat cells, U-937 cells, K-562 cells, RAW 264.7 cells, rat brain tissue
Positive IHC detected inmouse placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MOSPD2 Ready-To-Use IHC Kit. MOSPD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83398-1-RR targets MOSPD2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag22206

Product name: Recombinant human MOSPD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 64-496 aa of BC030641

Sequence: MLDESFQWRKEISVNDLNESSIPRWLLEIGVIYLHGYDKEGNKLFWIRVKYHVKDQKTILDKKKLIAFWLERYAKRENGKPVTVMFDLSETGINSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWLMNAAFKIVKTWLGPEAVSLLKFTSKNEVQDYVSVEYLPPHMGGTDPFKYSYPPLVDDDFQTPLCENGPITSEDETSSKEDIESDGKETLETISNEEQTPLLKKINPTESTSKAEENEKVDSKVKAFKKPLSVFKGPLLHISPAEELYFGSTESGEKKTLIVLTNVTKNIVAFKVRTTAPEKYRVKPSNSSCDPGASVDIVVSPHGGLTVSAQDRFLIMAAEMEQSSGTGPAELTQFWKEVPRNKVMEHRLRCHTVESSKPNTLTLKDNAFNMSDKTSEDICLQLSRLLESNRKLEDQVQRCIWFQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 motile sperm domain containing 2
别名 240282C11, Motile sperm domain-containing protein 2
计算分子量 518 aa, 60 kDa
观测分子量 59 kDa
GenBank蛋白编号BC030641
基因名称 MOSPD2
Gene ID (NCBI) 158747
RRIDAB_3671050
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NHP6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).

实验方案

Product Specific Protocols
FC protocol for MOSPD2 antibody 83398-1-RRDownload protocol
IHC protocol for MOSPD2 antibody 83398-1-RRDownload protocol
WB protocol for MOSPD2 antibody 83398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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