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验证数据展示

  • WB analysis of HEK-293 using 80064-2-RR

    WB analysis of HEK-293 using 80064-2-RR

    Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • WB analysis using 80064-2-RR

    WB analysis using 80064-2-RR

    Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-MEK6 (Ser207) Recombinant antibody

Phospho-MEK6 (Ser207) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80064-2-RR

产品说明书

CloneNo. 250293G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

MKK6 (Ser207), Phospho-MEK6, Phospho-MEK6(Ser207), Phospho-MKK3 (Ser189), Phospho-MKK3 (Ser189)/MKK6 (Ser207)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inUV treated HEK-293 cells, UV NIH/3T3 cells, UV treated NIH/3T3 cells

The antibody also detects the phosphorylated at Ser218 of the MEK3.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80064-2-RR targets Phospho-MEK6 (Ser207) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 6
别名 MKK6 (Ser207), Phospho-MEK6, Phospho-MEK6(Ser207), Phospho-MKK3 (Ser189), Phospho-MKK3 (Ser189)/MKK6 (Ser207)
计算分子量 334 aa, 37 kDa
观测分子量38 kDa, 40kDa
GenBank蛋白编号BC012009
基因名称 MEK6
Gene ID (NCBI) 5608
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52564
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.

实验方案

Product Specific Protocols
WB protocol for Phospho-MEK6 (Ser207) antibody 80064-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols