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验证数据展示

  • WB analysis using 68397-1-Ig

    WB analysis using 68397-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of K-562 using 68397-1-Ig

    WB analysis of K-562 using 68397-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATPBD4 Monoclonal antibody

ATPBD4 Monoclonal Antibody for WB, ELISA
Cat No. 68397-1-Ig

产品说明书

CloneNo. 2B12E1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

ATP binding domain 4, ATPBD4, DPH6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, K-562 cells, LNCaP cells, HeLa cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68397-1-Ig targets ATPBD4 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 ATPBD4 fusion protein Ag33218 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP binding domain 4
别名 ATP binding domain 4, ATPBD4, DPH6
计算分子量 267 aa, 30 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC008485
基因名称 ATPBD4
Gene ID (NCBI) 89978
RRIDAB_3085114
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7L8W6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

实验方案

Product Specific Protocols
WB protocol for ATPBD4 antibody 68397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols