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验证数据展示

  • WB analysis using 68374-1-Ig

    WB analysis using 68374-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (ESYT2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68374-1-Ig

    WB analysis of HeLa using 68374-1-Ig

    WB result of FAM62B antibody (68374-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells.

  • IF Staining of MCF-7 using 68374-1-Ig

    IF Staining of MCF-7 using 68374-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ESYT2 antibody (68374-1-Ig, Clone: 1B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  • KD/KO验证

ESYT2 Monoclonal antibody

ESYT2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68374-1-Ig

产品说明书

CloneNo. 1B12G2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HL-60 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FAM62B Ready-To-Use IHC Kit. FAM62B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68374-1-Ig targets ESYT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ESYT2 fusion protein Ag20440 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 62 (C2 domain containing) member B
别名 FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2
计算分子量 921 aa, 102 kDa
观测分子量90-100 kDa
GenBank蛋白编号BC013957
基因名称 ESYT2
Gene ID (NCBI) 57488
RRIDAB_3085093
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDA0FGR8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.

实验方案

Product Specific Protocols
WB protocol for ESYT2 antibody 68374-1-IgDownload protocol
IF protocol for ESYT2 antibody 68374-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols