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验证数据展示

  • WB analysis using 68285-1-Ig

    WB analysis using 68285-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68285-1-Ig (RABEP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68285-1-Ig

    IF Staining of HeLa using 68285-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 68285-1-Ig

    IF Staining of HeLa using 68285-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RABEP1 Monoclonal antibody

RABEP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68285-1-Ig

产品说明书

CloneNo. 1E7E11

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit, Pig

应用

WB, IF/ICC, ELISA

别名

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inpig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68285-1-Ig targets RABEP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig
免疫原

CatNo: Ag6023

Product name: Recombinant human RABEP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 561-862 aa of BC041700

Sequence: QLMLRQANDQLEKTMKDKQELEDFIKQSSEDSSHQISALVLRAQASEILLEELQQGLSQAKRDVQEQMAVLMQSREQVSEELVRLQKDNDSLQGKHSLHVSLQQAEDFILPDTTEALRELVLKYREDIINVRTAADHVEEKLKAEILFLKEQIQAEQCLKENLEETLQLEIENCKEEIASISSLKAELERIKVEKGQLESTLREKSQQLESLQEIKISLEEQLKKETAAKATVEQLMFEEKNKAQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQADSLERIRAILNDTKLTDINQLPET

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 rabaptin, RAB GTPase binding effector protein 1
别名 RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A
计算分子量 99 kDa
观测分子量100-105 kDa
GenBank蛋白编号BC041700
基因名称 RABEP1
Gene ID (NCBI) 9135
RRIDAB_2935366
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15276
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)

实验方案

Product Specific Protocols
IF protocol for RABEP1 antibody 68285-1-IgDownload protocol
WB protocol for RABEP1 antibody 68285-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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