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验证数据展示

  • WB analysis using 68284-1-Ig

    WB analysis using 68284-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68284-1-Ig (NEUROD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of mouse cerebellum using 68284-1-Ig

    WB analysis of mouse cerebellum using 68284-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68284-1-Ig (NEUROD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

NEUROD2 Monoclonal antibody

NEUROD2 Monoclonal Antibody for WB, ELISA
Cat No. 68284-1-Ig

产品说明书

CloneNo. 2C7B3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig

应用

WB, ELISA

别名

bHLHa1, NDRF, NeuroD related factor, NEUROD2, neurogenic differentiation 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig brain tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68284-1-Ig targets NEUROD2 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, pig
免疫原

CatNo: Ag26068

Product name: Recombinant human NEUROD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 314-382 aa of BC022481

Sequence: DSSPDHEKSYHYSMHYSALPGSRPTGHGLVFGSSAVRGGVHSENLLSYDMHLHHDRGPMYEELNAFFHN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neurogenic differentiation 2
别名 bHLHa1, NDRF, NeuroD related factor, NEUROD2, neurogenic differentiation 2
计算分子量 41 kDa
观测分子量41-50 kDa
GenBank蛋白编号BC022481
基因名称 NEUROD2
Gene ID (NCBI) 4761
RRIDAB_2935365
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15784
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEUROD2 (Neurogenic differentiation factor 2), is transcriptional regulator that regulates early neuronal differentiation during development (PMID: 7754368). NeuroD2 is expressed in the postnatal cortex and mediate the activity-dependent refinement of thalamocortical axon terminals (PMID: 17453014). NeuroD2 is mainly involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Mutantions in NEUROD2 can cause neurodevelopmental disorders including intellectual disability and autism spectrum disorders (PMID: 34188164).

实验方案

Product Specific Protocols
WB protocol for NEUROD2 antibody 68284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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