• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68157-1-Ig

    WB analysis using 68157-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68157-1-Ig

    WB analysis of HepG2 using 68157-1-Ig

    WB result of HIBCH antibody (68157-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells.

  • IF Staining of HepG2 using 68157-1-Ig

    IF Staining of HepG2 using 68157-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

HIBCH Monoclonal antibody

HIBCH Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68157-1-Ig

产品说明书

CloneNo. 1B7B8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, pig heart tissue, rabbit heart tissue, rat heart tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68157-1-Ig targets HIBCH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 HIBCH fusion protein Ag6473 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 3-hydroxyisobutyryl-Coenzyme A hydrolase
别名 HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase
计算分子量 43 kDa
观测分子量38 kDa
GenBank蛋白编号BC067822
基因名称 HIBCH
Gene ID (NCBI) 26275
RRIDAB_2935261
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6NVY1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

实验方案

Product Specific Protocols
WB protocol for HIBCH antibody 68157-1-IgDownload protocol
IF protocol for HIBCH antibody 68157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols