验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, LNCaP cells, Jurkat cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
68137-1-Ig targets TSPO/PBR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | TSPO/PBR fusion protein Ag7475 种属同源性预测 |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | translocator protein (18kDa) |
| 别名 | TSPO, Peripheral-type benzodiazepine receptor, MBR, BZRP, 1D1F12 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC001110 |
| 基因名称 | TSPO |
| Gene ID (NCBI) | 706 |
| RRID | AB_2923664 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | P30536 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPO/PBR antibody 68137-1-Ig | Download protocol |
| IF protocol for TSPO/PBR antibody 68137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |