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验证数据展示

  • WB analysis of mouse testis using 29758-1-AP

    WB analysis of mouse testis using 29758-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 29758-1-AP (DYNC2H1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 29758-1-AP

    IF Staining of hTERT-RPE1 using 29758-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IF Staining of hTERT-RPE1 using 29758-1-AP

    IF Staining of hTERT-RPE1 using 29758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IHC staining of mouse kidney using 29758-1-AP

    IHC staining of mouse kidney using 29758-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 29758-1-AP

    IHC staining of mouse kidney using 29758-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DYNC2H1 Polyclonal antibody

DYNC2H1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29758-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DHC1b, DHC2, DNCH2, DYH1B, DYNC2H1, FLJ11756, hdhc11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29758-1-AP targets DYNC2H1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 DYNC2H1 fusion protein Ag30707 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, cytoplasmic 2, heavy chain 1
别名 DHC1b, DHC2, DNCH2, DYH1B, DYNC2H1, FLJ11756, hdhc11
计算分子量 493 kDa
观测分子量493 kDa
GenBank蛋白编号NM_001377
基因名称 DYNC2H1
Gene ID (NCBI) 79659
RRIDAB_2935476
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NCM8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYNC2H1 (Cytoplasmic dynein 2 heavy chain 1) is involved in ciliary intraflagellar transport (IFT). DYNC2H1 drives retrograde transport of the IFT-A protein complex that regulates tip-to-base transport in cilia, involved in the generation and maintenance of cilia. DYNC2H1 variants or retina-predominant variants cause nonsyndromic retinal degeneration (PMID:32753734). DYNC2H1 mutations also cause asphyxiating thoracic dystrophy and short rib-polydactyly syndrome (PMID: 19442771). DYNC2H1 was localized to the basal bodies by immunofluorescence staining (PMID: 21552265, PMID: 26077881, PMID: 30320547).

实验方案

Product Specific Protocols
WB protocol for DYNC2H1 antibody 29758-1-APDownload protocol
IHC protocol for DYNC2H1 antibody 29758-1-APDownload protocol
IF protocol for DYNC2H1 antibody 29758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols