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验证数据展示

  • WB analysis using 29041-1-AP

    WB analysis using 29041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 29041-1-AP

    IF Staining of C2C12 using 29041-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse liver using 29041-1-AP

    IHC staining of mouse liver using 29041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29041-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CES1 antibody included
  • IF Staining of HepG2 using 29041-1-AP

    IF Staining of HepG2 using 29041-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CES1 Polyclonal antibody

CES1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29041-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, rat liver tissue, hepG2 cells, Caco-2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CES1 Ready-To-Use IHC Kit. CES1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29041-1-AP targets CES1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原

CatNo: Ag30534

Product name: Recombinant human CES1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 505-566 aa of BC012418

Sequence: NGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxylesterase 1 (monocyte/macrophage serine esterase 1)
别名 ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase
计算分子量 566 aa, 62 kDa
观测分子量62 kDa
GenBank蛋白编号BC012418
基因名称 CES1
Gene ID (NCBI) 1066
RRIDAB_3086096
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23141
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human carboxylesterase 1 (hCE1 or CES1) is a serine esterase that hydrolyzes a variety of endogenous and exogenous substrates. CES1 contributes to approximately 90% of hepatic hydrolytic activity in human livers (PMID: 27895113). Variation in CES1 has the ability to affect the metabolism of methylphenidate (PMID: 28087982). CES1 has 567 amino acids and a theoretical molecular mass of 62 kDa.

实验方案

Product Specific Protocols
IF protocol for CES1 antibody 29041-1-APDownload protocol
IHC protocol for CES1 antibody 29041-1-APDownload protocol
WB protocol for CES1 antibody 29041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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