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验证数据展示

  • WB analysis using 28456-1-AP

    WB analysis using 28456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28456-1-AP (HPF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 28456-1-AP

    IF Staining of A431 using 28456-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using HPF1 antibody (28456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HPF1 Polyclonal antibody

HPF1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28456-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Histone PARylation factor 1, C4orf27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28456-1-AP targets HPF1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HPF1 fusion protein Ag29377 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 4 open reading frame 27
别名 Histone PARylation factor 1, C4orf27
计算分子量 346 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC010367
基因名称 HPF1
Gene ID (NCBI) 54969
RRIDAB_3086053
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWY4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPF1 is also known as C4orf27 and belongs to the HPF1 family. HPF1 is a novel interacting component of PARP1 complex that plays an important role in PARP1-dependent ADP-ribosylation signaling in the DNA damage response and the maintenance of genome stability (PMID: 29549427). The calculated molecular weight of HPF1 is 39 kDa.

实验方案

Product Specific Protocols
WB protocol for HPF1 antibody 28456-1-APDownload protocol
IF protocol for HPF1 antibody 28456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols