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验证数据展示

  • IHC staining of rat testis using 27796-1-AP

    IHC staining of rat testis using 27796-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27796-1-AP (LRRC56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27796-1-AP

    IHC staining of mouse brain using 27796-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27796-1-AP (LRRC56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 27796-1-AP

    IF Staining of U-251 using 27796-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LRRC56 antibody (27796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

LRRC56 Polyclonal antibody

LRRC56 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 27796-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

leucine rich repeat containing 56

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27796-1-AP targets LRRC56 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LRRC56 fusion protein Ag22018 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 56
别名 leucine rich repeat containing 56
计算分子量 542 aa, 59 kDa
GenBank蛋白编号BC035936
基因名称 LRRC56
Gene ID (NCBI) 115399
RRIDAB_3669624
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYG6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine-rich repeat (LRR)-containing gene 56 (LRRC56) was recently reported to be a homolog of the Outer Dynein Arm 8 gene (oda8), a gene necessary for the cytoplasmic maturation of the outer dynein arms (ODA) in the flagella of algae. LRRC56 is recruited during axoneme construction in an IFT-dependent manner and is required for the addition of dynein arms to the distal segment of the flagellum. Human LRRC56 encodes a 542 amino acid protein determined from transcriptomic studies and immunohistochemistry to be expressed in many organs, mostly in testis and pituitary gland.

实验方案

Product Specific Protocols
IHC protocol for LRRC56 antibody 27796-1-APDownload protocol
IF protocol for LRRC56 antibody 27796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols