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验证数据展示

  • IHC staining of human colon cancer using 24804-1-AP

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24804-1-AP

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24804-1-AP

    WB analysis using 24804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24804-1-AP (STEAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

STEAP2 Polyclonal antibody

STEAP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24804-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells, HEK-293T cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:800-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24804-1-AP targets STEAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20293

Product name: Recombinant human STEAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 155-265 aa of BC148824

Sequence: QLGPKDASRQVYICSNNIQARQQVIELARQLNFIPIDLGSLSSAREIENLPLRLFTLWRGPVVVAISLATFFFLYSFVRDVIHPYARNQQSDFYKIPIEIVNKTLPIVAIT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 six transmembrane epithelial antigen of the prostate 2
别名 PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2
计算分子量 490 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC148824
基因名称 STEAP2
Gene ID (NCBI) 261729
RRIDAB_3085755
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.

实验方案

Product Specific Protocols
IHC protocol for STEAP2 antibody 24804-1-APDownload protocol
WB protocol for STEAP2 antibody 24804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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