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验证数据展示

  • IHC staining of mouse brain using 24528-1-AP

    IHC staining of mouse brain using 24528-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24528-1-AP (SYTL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24528-1-AP

    IHC staining of mouse brain using 24528-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24528-1-AP (SYTL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 24528-1-AP

    IF Staining of rat brain using 24528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SYTL5 antibody (24528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SYTL5 Polyclonal antibody

SYTL5 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 24528-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, ELISA

别名

slp5, synaptotagmin like 5, Synaptotagmin like protein 5, SYTL5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24528-1-AP targets SYTL5 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21698

Product name: Recombinant human SYTL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 56-343 aa of BC131585

Sequence: TQQEASRVCVHCHRNLGLIFDRGDPCQACSLRVCRECRVAGPNGSWKCTVCDKIAQLRIITGEWFFEEKAKRFKQVNVLGTDVVRQSILRRSPGAEEVQSQEQTRQDAEKSDTSPVAGKKASHDGPKRKGFLLSKFRSATRGEIITPKTDTGRSYSLDLDGQHFRSLKSPPGSDRGSTGSSDLNDQEPGPRTPKSSRSNGVTPGTQSSPAPSTRTVTSVISREYGFENSMDLAAIEGTSQELTKSHRRNTSGTPSIAVSGTSLSSDQSRSELDLSESFTEDSEDTVSI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptotagmin-like 5
别名 slp5, synaptotagmin like 5, Synaptotagmin like protein 5, SYTL5
计算分子量 730 aa, 81 kDa
GenBank蛋白编号BC131585
基因名称 SYTL5
Gene ID (NCBI) 94122
RRIDAB_3669441
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYTL5, also known as SLP5, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by the presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL5 promotes the development and progression of papillary thyroid carcinoma (PTC) by activating the NF-κB signaling pathway (PMID: 36378561).

实验方案

Product Specific Protocols
IF protocol for SYTL5 antibody 24528-1-APDownload protocol
IHC protocol for SYTL5 antibody 24528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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