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验证数据展示

  • WB analysis using 24425-1-AP

    WB analysis using 24425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24425-1-AP

    WB analysis using 24425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24425-1-AP

    WB analysis using 24425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24425-1-AP

    IF Staining of HepG2 using 24425-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCMTD1 antibody (24425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PCMTD1 Polyclonal antibody

PCMTD1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24425-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1, Protein L isoaspartate O methyltransferase domain containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat liver tissue, human placenta tissue, hTERT-RPE1 cells, mouse eye tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24425-1-AP targets PCMTD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PCMTD1 fusion protein Ag19634 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1
别名 Protein-L-isoaspartate O-methyltransferase domain-containing protein 1, Protein L isoaspartate O methyltransferase domain containing protein 1
计算分子量 357 aa, 41 kDa
观测分子量41 kDa, 27 kDa
GenBank蛋白编号BC032670
基因名称 PCMTD1
Gene ID (NCBI) 115294
RRIDAB_3669436
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MG8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein-l-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1) is a putative E3 ubiquitin ligase substrate adaptor protein, which has a C-terminal domain containing SOCS-box ubiquitin ligase recruitment motifs. PCMTD1 protein may provide an alternate maintenance pathway for modified proteins in mammalian cells (PMID: 35486881). This antibody can detect two isoforms of PCMTD1, 27 kDa and 41 kDa respectively (PMID: 37953789, 35486881).

实验方案

Product Specific Protocols
WB protocol for PCMTD1 antibody 24425-1-APDownload protocol
IF protocol for PCMTD1 antibody 24425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols