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验证数据展示

  • WB analysis of human plasma using 23029-1-AP

    WB analysis of human plasma using 23029-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 23029-1-AP

    IP experiment of human plasma using 23029-1-AP

    IP result of anti-PGLYRP2 (IP:23029-1-AP, 4ug; Detection:23029-1-AP 1:1000) with human plasma lysate 4000ug.

  • IHC staining of human liver cancer using 23029-1-AP

    IHC staining of human liver cancer using 23029-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 23029-1-AP

    IHC staining of human liver cancer using 23029-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:50 (under 40x lens).

PGLYRP2 Polyclonal antibody

PGLYRP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 23029-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

HMFT0141, PGLYRP2, PGLYRPL, PGRP L, PGRPL, tagL, tagL alpha, tagl beta, TAGL like

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23029-1-AP targets PGLYRP2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19336

Product name: Recombinant human PGLYRP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-369 aa of BC136551

Sequence: LLMDSVIQALAELEQKVPAAKTRHTASAWLMSAPNSGPHNRLYHFLLGAWSLNATELDPCPLSPELLGLTKEVARHDVREGKEYGVVLAPDGSTVAVEPLLAGLEAGLQGRRVINLPLDSMAAPWETGDTFPDVVAIAPDVRATSSPGLRDGSPDVTTADIGANTPDATKGCPDVQASLPDAKAKSPPTMVDSLLAVTLAGNLGLTFLRGSQTQSHPDLGTEGCWDQLSAPRTFTLLDPKASLLTMAFLNGALDGVILGDYLSRTPEPRPSLSHLLSQYYGAGVARDPGFRSNFRRQNGAALTSASILAQQVWGTLVLLQRLEPVHLQLQCMSQEQLAQVAANAT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidoglycan recognition protein 2
别名 HMFT0141, PGLYRP2, PGLYRPL, PGRP L, PGRPL, tagL, tagL alpha, tagl beta, TAGL like
计算分子量 576 aa, 62 kDa
观测分子量 68 kDa
GenBank蛋白编号BC136551
基因名称 PGLYRP2
Gene ID (NCBI) 114770
RRIDAB_2879199
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96PD5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidoglycan recognition protein 2 (PGLYRP2) is an N-acetylmuramoyl-L-alanine amidase that hydrolyzes peptidoglycan, a ubiquitous component of bacterial cell walls (PMID: 14506276). It is involved in innate immunity. PGLYRP2 is primarily expressed in liver and secreted into blood (PMID: 11461926). The apparent molecular mass measured by SDS-PAGE is lager than calculated mass of 62 kDa, which is likely due to N-glycosylation (PMID: 16054449).

实验方案

Product Specific Protocols
IHC protocol for PGLYRP2 antibody 23029-1-APDownload protocol
IP protocol for PGLYRP2 antibody 23029-1-APDownload protocol
WB protocol for PGLYRP2 antibody 23029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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