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- KD/KO Validated
PSD95-Specific,DLG4 Polyclonal antibody
PSD95-Specific,DLG4 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
FC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
161
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | Neuro-2a cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20665-1-AP targets PSD95-Specific,DLG4 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IHC, WB, ELISA |
Cited Applications | WB, IF, FC, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish, hamster |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | discs, large homolog 4 (Drosophila) |
Synonyms | Disks large homolog 4, DLG4, PSD 95, PSD95, PSD95-Specific,DLG4, SAP 90, SAP90, Synapse associated protein 90 |
Calculated Molecular Weight | 85 kDa |
Observed Molecular Weight | 90-95 kDa |
GenBank Accession Number | NM_001365 |
Gene Symbol | DLG4 |
Gene ID (NCBI) | 1742 |
RRID | AB_2687961 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P78352 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PSD95-Specific,DLG4 antibody 20665-1-AP | Download protocol |
IHC protocol for PSD95-Specific,DLG4 antibody 20665-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Neurosci Foxp2 controls synaptic wiring of corticostriatal circuits and vocal communication by opposing Mef2c. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Neuron Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia. | ||
Sci Adv Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission | ||
Sci Adv RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission | ||
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. |