验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells |
| Positive IHC detected in | human cervical cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
19690-1-AP targets EIF2C1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2C, 1 |
| 别名 | AGO1, Argonaute1, eIF 2C 1, EIF2C, eIF2C 1, EIF2C1, EIF2C1-Specific, GERP95, hAgo1, Protein argonaute 1, Q99 |
| 计算分子量 | 97 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | NM_012199 |
| 基因名称 | EIF2C1 |
| Gene ID (NCBI) | 26523 |
| RRID | AB_10863657 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL18 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2C1-Specific antibody 19690-1-AP | Download protocol |
| IHC protocol for EIF2C1-Specific antibody 19690-1-AP | Download protocol |
| IF protocol for EIF2C1-Specific antibody 19690-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Mol Neurosci miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif. | ||
Mol Ther Oncolytics Hsa_circ_0001666 promotes non-small cell lung cancer migration and invasion through miR-1184/miR-548I/AGO1 axis. | ||
