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验证数据展示

  • WB analysis using 16378-1-AP

    WB analysis using 16378-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16378-1-AP (MRPS28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16378-1-AP

    IHC staining of human liver using 16378-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16378-1-AP (MRPS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MRPS28 antibody included
  • IF Staining of HeLa using 16378-1-AP

    IF Staining of HeLa using 16378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS28 antibody (16378-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MRPS28 Polyclonal antibody

MRPS28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16378-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MRPS35, MRP-S28, MRPS 28, MRP S35, MRP S28

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MRPS28 Ready-To-Use IHC Kit. MRPS28 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16378-1-AP targets MRPS28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MRPS28 fusion protein Ag9354 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S28
别名 MRPS35, MRP-S28, MRPS 28, MRP S35, MRP S28
计算分子量 187 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC010150
基因名称 MRPS28
Gene ID (NCBI) 28957
RRIDAB_2180748
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2Q9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS28 antibody 16378-1-APDownload protocol
IHC protocol for MRPS28 antibody 16378-1-APDownload protocol
IF protocol for MRPS28 antibody 16378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Authors - Juliette Pulman
View Article