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验证数据展示

  • WB analysis using 15575-1-AP

    WB analysis using 15575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15575-1-AP (GMCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid using 15575-1-AP

    IHC staining of human thyroid using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

  • IHC staining of human thyroid using 15575-1-AP

    IHC staining of human thyroid using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

GMCL1 Polyclonal antibody

GMCL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15575-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SPATA29, Germ cell-less protein-like 1, Germ cell less protein like 1, GCL1, GCL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman thyroid tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15575-1-AP targets GMCL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GMCL1 fusion protein Ag7929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 germ cell-less homolog 1 (Drosophila)
别名 SPATA29, Germ cell-less protein-like 1, Germ cell less protein like 1, GCL1, GCL
计算分子量 59 kDa
观测分子量 55 kDa
GenBank蛋白编号BC007420
基因名称 GMCL1
Gene ID (NCBI) 64395
RRIDAB_2878153
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96IK5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMCL1, also named as GCL, functions in spermatogenesis. It enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport. In WB test, the antibody recognizes 55 kDa band in our detection.

实验方案

Product Specific Protocols
WB protocol for GMCL1 antibody 15575-1-APDownload protocol
IHC protocol for GMCL1 antibody 15575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols