验证数据展示
经过测试的应用
| Positive WB detected in | human thyroid gland tissue, human brain tissue, mouse embryo tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
11844-1-AP targets UBE2E2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2509 Product name: Recombinant human UBE2E2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC022332 Sequence: MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) |
| 别名 | Ubiquitin-conjugating enzyme E2 E2, Ubiquitin protein ligase E2, Ubiquitin carrier protein E2, UBCH8, EC:2.3.2.23 |
| 计算分子量 | 201 aa, 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC022332 |
| 基因名称 | UBE2E2 |
| Gene ID (NCBI) | 7325 |
| RRID | AB_2210274 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96LR5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2E2 antibody 11844-1-AP | Download protocol |
| IHC protocol for UBE2E2 antibody 11844-1-AP | Download protocol |
| IF protocol for UBE2E2 antibody 11844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Sequential replication-coupled destruction at G1/S ensures genome stability.
| ||
Cell Death Dis UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer
| ||
Mol Cell Biol Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex. | ||
