Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

1X10^6 A549 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human PLK1 (CL555-10305) (red), or 0.2 ug CL555 Rabbit Isotype Control Antibody(CL555-30000)(blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PLK1 (CL647-10305) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC1624 (PLK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using KHC1624 (PLK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using KHC1624 (PLK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using KHC1624 (PLK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using KHC1624 (PLK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using KHC1624 (PLK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using KHC1624 (PLK1 IHC Kit).

Cytometric bead array standard curve ofMP00238-1, PLK1-phospho Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83260-2-PBS. Detection antibody: 83260-3-PBS. Standard: SY00401. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve ofMP00238-2, PLK1-phospho Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83260-2-PBS. Detection antibody: 83260-4-PBS. Standard: SY00401. Range: 0.313-10 ng/mL.

Cytometric bead array standard curve ofMP00238-1, PLK1-phospho Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83260-2-PBS. Detection antibody: 83260-3-PBS. Standard: SY00401. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve ofMP00238-2, PLK1-phospho Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83260-2-PBS. Detection antibody: 83260-4-PBS. Standard: SY00401. Range: 0.313-10 ng/mL.

Standard curve of MP00238-1