Various lysates were subjected to SDS PAGE followed by western blot with 18722-1-AP (LC3A-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of HepG2 cells, using MAP1LC3A antibody 18722-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18722-1-AP (LC3A-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18722-1-AP (LC3A-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HeLa cells were stained with 0.2ug LC3A-Specific antibody (18722-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
