The HA-Trap Agarose Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

Co-IP using HA-Trap Agarose Kit followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.

The HA-Trap Agarose (left) and a competitor resin (right) were used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. Immunoprecipitation with HA-Trap Agarose results in clean, single-band pulldowns without any heavy and light chain contamination. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

Co-IP using HA-Trap Agarose followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.

The HA-Trap Agarose was used to immunoprecipitate HA-PCNA and PCNA-HA proteins from transfected HEK293T cells. WB analysis was done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions of the IP using PCNA Monclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001). The HA-Trap is succesful in pulling down HA-tagged PCNA regardless of whether the tag is fused to the N- or C-terminal. Note: PCNA forms trimers, resulting in co-elution of endogenous PCNA with HA-tagged PCNA.

The HA-Trap Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution with HA-peptide (ap). Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms trimers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

The HA-Trap Magnetic Agarose (left) and a competitor resin (right) were used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. Immunoprecipitation with HA-Trap Magnetc Agarose results in cleaner, single-band pulldowns without any heavy and light chain contamination. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

Co-IP using HA-Trap Magnetic Agarose followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.

The HA-Trap Magnetic Agarose was used to immunoprecipitate HA-PCNA and PCNA-HA proteins from transfected HEK293T cells. WB analysis was done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions of the IP using PCNA Monclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001). The HA-Trap is succesful in pulling down HA-tagged PCNA regardless of whether the tag is fused to the N- or C-terminal. Note: PCNA forms trimers, resulting in co-elution of endogenous PCNA with HA-tagged PCNA.

The HA-Trap Magnetic Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide (ap). Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

The HA-Trap Magnetic Agarose Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

Co-IP using HA-Trap Magnetic Agarose Kit followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.

The HA-Trap Magnetic Particles M-270 (left) and a competitor resin (right) were used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. Immunoprecipitation with HA-Trap Magnetc Particles M-270 results in cleaner, single-band pulldowns without any heavy and light chain contamination. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

WB detection of TOM70-HA fusion protein following immunoprecipitation with HA-Trap Magnetic Particles M-270 from either untransfected (mock) HEK293T cells or HEK293T cells transfected with full-length TOM70-HA construct. Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the WB analysis. Detection was completed using TOM70 Monoclonal Antibody (66593-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001).

The HA-Trap Magnetic Particles M-270 was used to immunoprecipitate HA-PCNA and PCNA-HA proteins from transfected HEK293T cells. WB analysis was done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions of the IP using PCNA Monclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001). The HA-Trap is succesful in pulling down HA-tagged PCNA regardless of whether the tag is fused to the N- or C-terminal. Note: PCNA forms trimers, resulting in co-elution of endogenous PCNA with HA-tagged PCNA.

The HA-Trap Magnetic Particles M-270 was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide (ap). Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

The HA-Trap Magnetic Particles M-270 Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

WB detection of TOM70-HA fusion protein following immunoprecipitation with HA-Trap Magnetic Particles M-270 Kit from either untransfected (mock) HEK293T cells or HEK293T cells transfected with full-length TOM70-HA construct. Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the WB analysis. Detection was completed using TOM70 Monoclonal Antibody (66593-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001).

Structure of HA-tag

The HA-Trap Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution with HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms trimers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

The HA-Trap Magnetic Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.

The HA-Trap Magnetic Particles M-270 was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.