ubiquitin Polyclonal antibody

ubiquitin Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)


FC, IHC, WB, ELISA and More (3)



Cat No : 10201-2-AP

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Polyubiquitin B, UBB, ubiquitin, ubiquitin B


Positive WB detected inMDA-MB-453s cells, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells


Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery


10201-2-AP targets ubiquitin in WB, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications ChIP, CoIP, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Arabidopsis, monkey, Trypanosoma cruzi
Immunogen ubiquitin fusion protein Ag0260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin B
Synonyms Polyubiquitin B, UBB, ubiquitin, ubiquitin B
Calculated molecular weight 26 kDa
GenBank accession numberBC000379
Gene symbol UBB
Gene ID (NCBI) 7314
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.


Product Specific Protocols
WB protocol for ubiquitin antibody 10201-2-APDownload protocol
IHC protocol for ubiquitin antibody 10201-2-APDownload protocol
FC protocol for ubiquitin antibody 10201-2-APDownload protocol
Standard Protocols
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