ChromoTek pan-RFP Polyclonal antibody

Rabbit polyclonal antibody to pan-Red Fluorescent Protein
Cat No. pabr1

Host/IsoType

Rabbit / IgG

Specificity

RFP and RFP derivates

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Red fluorescent protein, RFP, mRFP, dsRed, mCherry, tdTomato, mScarlet, TagRFP, TagBFP, mKate, mRuby

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Product Information

Rabbit polyclonal antibody to pan-Red Fluorescent Protein

DescriptionRabbit polyclonal antibody to Red Fluorescent Protein
ApplicationsWB, IF, ELISA
ReactivityRecombinant proteins, stably and transiently transfected cell lines; red fluorescent proteins from Discosoma (dsRed, mCherry, tdTomato, mPlum); fluorescent proteins from Entacmaea lineages (TagRFP, TagBFP, mKate2, mRuby2), synthetic red fluorescent protein mScarlet.
Specificity/TargetTagBFP, DsRed, mCherry, mRuby2, mScarlet, TagRFP, tdTomato, mKate2, mPlum
Recommended DilutionWestern blot: 1:1,000
IF: 1:400-1:800
ELISA: N/A
Host/IsoTypeRabbit / IgG
ClassPolyclonal
TypePrimary Antibody
ImmunogenRed Fluorescent Proteins (RFPs), a mixture of several representatives from different lineages
Calculated molecular weight26kDa
ConjugateUnconjugated
FormLiquid
Purification MethodAntigen affinity purification
RRIDAB_3101915
Storage BufferPBS with 50% glycerol and 0.05% NaN3
Storage ConditionShipped on ice. Store at -20°C. Stable for 1 year after shipment.
Size20 µL; 150 µL
BackgroundRed fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.
The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).
When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
PABR1 is a rabbit polyclonal antibody raised against a mixture of RFP and can detect a broad range of RFPs from different species. This antibody detects dsRed-derivatives from Discosoma sp., including mCherry, mRFP, tdTomato, mPlum.
PABR1 antibody also detects representatives of different FP lineages from Entacmaea sp., such as eqFP578-derivatives including TagRFP, TagBFP, mKate2, as well as eqFP611-derivatives including mRuby2. PABR1 also detects the red fluorescent protein mScarlet, engineered from a synthetic template.