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验证数据展示

  • WB analysis of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    WB analysis of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCTN1 antibody included
  • IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCTN1 antibody included
  • WB analysis of HeLa using 55182-1-AP (same clone as 55182-1-PBS)

    WB analysis of HeLa using 55182-1-AP (same clone as 55182-1-PBS)

    WB result of DCTN1/p150 glued antibody (55182-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCTN1/p150 glued transfected HeLa cells. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 55182-1-AP (same clone as 55182-1-PBS)

    IF Staining of MDCK using 55182-1-AP (same clone as 55182-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DCTN1/p150 glued Polyclonal antibody, PBS Only

DCTN1/p150 glued Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55182-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DCTN1, p150 glued, DP-150, DCTN 1, DAP-150

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DCTN1 Ready-To-Use IHC Kit. DCTN1 primary antibody included.

产品信息

55182-1-PBS targets DCTN1/p150 glued in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, canine
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynactin 1 (p150, glued homolog, Drosophila)
别名 DCTN1, p150 glued, DP-150, DCTN 1, DAP-150
计算分子量 142 kDa
观测分子量 135 kDa, 150 kDa
GenBank蛋白编号NM_004082
基因名称 p150 glued
Gene ID (NCBI) 1639
RRIDAB_10837074
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14203
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.