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Recombinant Mouse IL-2 protein (hFc Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

hFc Tag

生物活性

EC50: 5-20 ng/mL

Cat no : Eg0456

Print datasheet

Synonyms

Il2, IL-2, Il 2, interleukin 2, Interleukin-2

验证数据展示

  • Purity of Recombinant Mouse IL-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 5-20 ng/mL.

    Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 5-20 ng/mL.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 5-20 ng/mL.
来源 HEK293-derived Mouse IL-2 protein Met+Ala21-Gln169 (Accession# P04351) with a human IgG1 Fc tag at the N-terminus.
基因ID 16183
蛋白编号 P04351
预测分子量 43.5 kDa
SDS-PAGE 45-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

IL-2 (Interleukin-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells. IL-2 mediates its effects by binding to IL-2 receptors which are expressed by lymphocytes. IL-2 signal can be transduced by 3 different signaling pathways: JAK/STAT, PI3K/Akt/mTOR and MAPK/ERK pathway. IL-2 plays a role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. It also promotes proliferation of activated B cells and subsequently immunoglobulin production.

参考文献:

1.M C Mingari. et al. (1984). Nature. 312(5995): 641-3.
2.Thomas R Malek. et al. (2010). Immunity. 33(2): 153-65.
3.Wei Liao. et al. (2011). Curr Opin Immunol. 23(5): 598-604.
4.Natalia Arenas Ramirez. et al. (2015). Trends Immunol. 36(12): 763-777.

Publications

SpeciesTitle

Biochem Biophys Res Commun

Immune escape between endoplasmic reticulum stress-related cancer cells and exhausted CD8+T cells leads to neoadjuvant chemotherapy resistance in ovarian cancer

Authors - Siyang Zhang
View Article