- Featured Product
- KD/KO Validated
Lamin A/C Polyclonal antibody
Lamin A/C Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
FC, IF, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
155
验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HeLa cells, HUVEC cells, HEK-293 cells, A375 cells, C6 cells, mouse ovary tissue, SKOV-3 cells |
| Positive IP detected in | A375 cells |
| Positive IF detected in | HepG2 cells, HeLa cells |
| Positive FC detected in | HEK-293T cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
10298-1-AP targets Lamin A/C in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF, IP, WB, ELISA |
| Cited Applications | IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, duck, monkey |
| Immunogen | Lamin A/C fusion protein Ag0408 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lamin A/C |
| Synonyms | 70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 65 kDa, 70 kDa |
| GenBank accession number | BC003162 |
| Gene symbol | LMNA |
| Uniprot | P02545 |
| Gene ID (NCBI) | 4000 |
| RRID | AB_2296961 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Lamin A/C antibody 10298-1-AP | Download protocol |
| IF protocol for Lamin A/C antibody 10298-1-AP | Download protocol |
| IP protocol for Lamin A/C antibody 10298-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Cancer Cell SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Sci Adv Interplay of m6A and H3K27 trimethylation restrains inflammation during bacterial infection. | ||
Nat Commun Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63. |
