|Positive WB detected in||NIH/3T3 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, A375 cells, C6 cells, HeLa cells|
|Positive IP detected in||A375 cells|
|Positive IF detected in||HepG2 cells, HeLa cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10298-1-AP targets Lamin A/C in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||FC, IF, IP, WB, ELISA|
|Cited Applications||IF, IP, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, duck|
|Immunogen||Lamin A/C fusion protein Ag0408|
|Host / Isotype||Rabbit / IgG|
|Full Name||lamin A/C|
|Synonyms||70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa, 70 kDa|
|GenBank accession number||BC003162|
|Gene ID (NCBI)||4000|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
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