验证数据展示

WB analysis using 10298-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10298-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 10298-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IP experiment of HeLa using 10298-1-AP
IP result of anti-Lamin A/C (IP:10298-1-AP, 4ug; Detection:10298-1-AP 1:50000) with HeLa cells lysate 1360 ug.
WB analysis of HeLa using 10298-1-AP
WB result of Lamin A/C antibody (10298-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells.
IHC staining of mouse heart using 10298-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Lamin A/C antibody included
IHC staining of mouse heart using 10298-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Lamin A/C antibody included
IHC staining of human normal colon using 10298-1-AP
Immunohistochemical analysis of paraffin-embedded normal colon slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:7000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Lamin A/C antibody included
IF Staining of HepG2 using 10298-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin A/C antibody (10298-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HEK-293T using 10298-1-AP
1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Lamin A/C (10298-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO验证

Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10298-1-AP

产品说明书

引用文献(251)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

LaminA/C, lamin A, Prelamin-A/C, LMNA, LMN1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (251)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, HeLa cells, A375 cells, C6 cells, NIH/3T3 cells
Positive IP detected in	HeLa cells, A375 cells
Positive IHC detected in	mouse heart tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HEK-293T cells

Planning an IHC experiment? We recommend our IHCeasy Lamin A/C Ready-To-Use IHC Kit. Lamin A/C primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 232 publications below
IHC	See 2 publications below
IF	See 15 publications below
IP	See 4 publications below
CoIP	See 1 publications below

产品信息

10298-1-AP targets Lamin A/C in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, monkey, duck
免疫原	Lamin A/C fusion protein Ag0408 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	lamin A/C
别名	LaminA/C, lamin A, Prelamin-A/C, LMNA, LMN1
计算分子量	65 kDa
观测分子量	65 kDa, 70 kDa
GenBank蛋白编号	BC003162
基因名称	Lamin A/C
Gene ID (NCBI)	4000
ENSEMBL Gene ID	ENSG00000160789
RRID	AB_2296961
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P02545
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

实验方案

Product Specific Protocols
WB protocol for Lamin A/C antibody 10298-1-AP	Download protocol
IHC protocol for Lamin A/C antibody 10298-1-AP	Download protocol
IF protocol for Lamin A/C antibody 10298-1-AP	Download protocol
IP protocol for Lamin A/C antibody 10298-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer Authors - Rashmi Bharti View Article
human	IF	Nat Microbiol Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus. Authors - Adarsh Dharan View Article
human	WB	Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. Authors - Jia Xiong View Article
human	WB	J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. Authors - Xiaohan Gao View Article
human	WB	Nat Chem Biol E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells Authors - Siqi Shen View Article
human	WB	Cancer Cell SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. Authors - Lan Fang View Article

验证数据展示

WB analysis using 10298-1-AP

WB analysis using 10298-1-AP

IF Staining of HepG2 using 10298-1-AP

IP experiment of HeLa using 10298-1-AP

WB analysis of HeLa using 10298-1-AP

IHC staining of mouse heart using 10298-1-AP

IHC staining of mouse heart using 10298-1-AP

IHC staining of human normal colon using 10298-1-AP

IF Staining of HepG2 using 10298-1-AP

FC experiment of HEK-293T using 10298-1-AP

Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.

Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells

SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.