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验证数据展示

  • WB analysis using 31495-1-AP

    WB analysis using 31495-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31495-1-AP (IGHG3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 31495-1-AP

    IHC staining of human tonsillitis using 31495-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31495-1-AP (human IgG3 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 31495-1-AP

    IHC staining of human tonsillitis using 31495-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31495-1-AP (human IgG3 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

human IgG3 Polyclonal antibody

human IgG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31495-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

IGHG3, IgG3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig spleen tissue, pig tonsil tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31495-1-AP targets human IgG3 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag35740

Product name: Recombinant human IGHG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 239-312 aa of NG_001019.6

Sequence: LHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin heavy constant gamma 3 (G3m marker)
别名 IGHG3, IgG3
计算分子量49kDa，446aa
观测分子量50-55 kDa
GenBank蛋白编号NG_001019.6
基因名称 IGHG3
Gene ID (NCBI) 3502
RRIDAB_3670007
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP01860
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for human IgG3 antibody 31495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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