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验证数据展示

  • WB analysis using 32186-1-AP

    WB analysis using 32186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32186-1-AP (gp130/IL6ST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

gp130/IL6ST Polyclonal antibody

gp130/IL6ST Polyclonal Antibody for WB, ELISA
Cat No. 32186-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

IL-6R-beta, IL-6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inC6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32186-1-AP targets gp130/IL6ST in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 6 signal transducer
别名 IL-6R-beta, IL-6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130
计算分子量102kDa, 917aa
观测分子量130 kDa
GenBank蛋白编号NM_010560.3
基因名称 Il6st
Gene ID (NCBI) 16195
RRIDAB_3670217
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ00560
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines(PMID: 39414364), including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin (PMID: 24099136). This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells. GP130 was originally discovered in 1978 and was successfully cloned and expressed in 1990, leading to a deeper understanding of cytokine signaling pathways (PMID: 308233).

实验方案

Product Specific Protocols
WB protocol for gp130/IL6ST antibody 32186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols