验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31658-1-AP targets dysferlin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35810 Product name: Recombinant human dysferlin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 60-210 aa of NM_001130455 Sequence: DQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDEAEPFLDQSGGPGAPTTPRKLPSRPPPHYPGIKRKRSAPTSR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) |
| 别名 | DYSF, LGMD2B, LGMD 2B, FER1L1, Fer 1 like protein 1 |
| 计算分子量 | 237 kDa |
| 观测分子量 | 240 kDa |
| GenBank蛋白编号 | NM_001130455 |
| 基因名称 | DYSF |
| Gene ID (NCBI) | 8291 |
| RRID | AB_3670065 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75923 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dysferlin, also known as FER1L1, belongs to the ferlin family and is a member of a putative muscle-specific repair complex. It has many isoforms and is a large membrane protein that is most abundant in striated muscle. It plays a role in sarcolemmal repair and is a key calcium ion sensor involved in Ca(2+)-triggered synaptic vesicle-plasma membrane fusion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for dysferlin antibody 31658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |