- Featured Product
- KD/KO Validated
Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (6)
Applications
FC, IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
565
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, MCF-7 cells, human placenta tissue, mouse lung tissue, HeLa cells, SH-SY5Y cells, HEK-293 cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue, mouse colon tissue, human liver cancer tissue, mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, T-47D cells, mouse testis tissue, HeLa cells, HepG2 cells |
| Positive FC detected in | MCF-7 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
51067-2-AP targets Beta Catenin in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | chIP, CoIP, IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, bovine, canine, chicken, goat, hamster, pig, sheep |
| Immunogen | Peptide |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Synonyms | b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin |
| Calculated molecular weight | 781 aa, 86 kDa |
| Observed molecular weight | 92 kDa |
| GenBank accession number | BC058926 |
| Gene symbol | CTNNB1 |
| Uniprot | P35222 |
| Gene ID (NCBI) | 1499 |
| RRID | AB_2086128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IHC protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IF protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IP protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hepatology Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress. | ||
Hepatology Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1. | ||
Nat Commun CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis. | ||
EMBO Mol Med Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. |
