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验证数据展示

  • WB analysis of HeLa using 51067-2-AP

    WB analysis of HeLa using 51067-2-AP

    WB result of Beta Catenin antibody (51067-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Catenin transfected HeLa cells.

  • WB analysis using 51067-2-AP

    WB analysis using 51067-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51067-2-AP (Beta Catenin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 51067-2-AP

    IHC staining of mouse liver using 51067-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of mouse liver using 51067-2-AP

    IHC staining of mouse liver using 51067-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IF Staining of MCF-7 using 51067-2-AP

    IF Staining of MCF-7 using 51067-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse liver using 51067-2-AP

    IP experiment of mouse liver using 51067-2-AP

    IP result of anti-Beta Catenin (IP:51067-2-AP, 4ug; Detection:51067-2-AP 1:500) with mouse liver tissue lysate 6400ug.

  • IF Staining of mouse testis using 51067-2-AP

    IF Staining of mouse testis using 51067-2-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 51067-2-AP

    FC experiment of MCF-7 using 51067-2-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Beta Catenin (51067-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using 51067-2-AP

    IF Staining of MCF-7 using 51067-2-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Featured Product
  • KD/KO验证

Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 51067-2-AP

产品说明书

引用文献(893)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig and More (6)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (3)

别名

b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293T cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse liver tissue, human colon cancer tissue, human liver cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue, mouse epididymis tissue
Positive IF/ICC detected inMCF-7 cells, HepG2 cells, HeLa cells, T-47D cells, mouse testis tissue
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51067-2-AP targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, canine, chicken, zebrafish, hamster, sheep, goat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1
计算分子量 781 aa, 86 kDa
观测分子量 92 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2086128
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
WB protocol for Beta Catenin antibody 51067-2-APDownload protocol
IHC protocol for Beta Catenin antibody 51067-2-APDownload protocol
IF protocol for Beta Catenin antibody 51067-2-APDownload protocol
IP protocol for Beta Catenin antibody 51067-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP,IHC

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
View Article
humanIF

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
View Article
humanWB

Hepatology

Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.

Authors - Meng Lu
View Article
mouse,ratWB

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
View Article
mouseWB

Hepatology

Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.

Authors - Sheng-Xian Yuan
View Article
zebrafishIF

Nat Commun

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.

Authors - Xiaoyun Sun
View Article