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验证数据展示

  • WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

    WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

    PC-3(AR-), DU 145(AR-) and LNCaP (AR+) cell lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AR antibody included
  • IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 66747-1-Ig (same clone as 66747-1-PBS)

    IF Staining of LNCaP using 66747-1-Ig (same clone as 66747-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

androgen receptor Monoclonal antibody, PBS Only

androgen receptor Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66747-1-PBS

产品说明书

CloneNo. 1F7C12
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA and More (1)

别名

androgen receptor,AR, AR, 1F7C12, DHTR, NR3C4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AR Ready-To-Use IHC Kit. AR primary antibody included.

发表文章中的应用

ChIPSee 1 publications below

产品信息

66747-1-PBS targets androgen receptor in WB, IHC, IF/ICC, IF-P, ChIP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
文献引用应用ChIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag17291

Product name: Recombinant human AR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-285 aa of BC132975

Sequence: MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 androgen receptor
别名 androgen receptor,AR, AR, 1F7C12, DHTR, NR3C4
计算分子量 914 aa, 99 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC132975
基因名称 AR
Gene ID (NCBI) 367
RRIDAB_2882094
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10275
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )

发表文章

SpeciesApplicationTitle
humanChIP

Sci Rep

Characterization of RNA editing gene APOBEC3C as a candidate tumor suppressor in prostate cancer

Authors - Li-Yang Wang
View Article