验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25956-1-AP targets ZNF644 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ZNF644 fusion protein Ag22798 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 644 |
| 别名 | ZEP2, Zep 2 |
| 计算分子量 | 1327 aa, 150 kDa |
| 观测分子量 | 175 kDa |
| GenBank蛋白编号 | BC063683 |
| 基因名称 | ZNF644 |
| Gene ID (NCBI) | 84146 |
| RRID | AB_3669512 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H582 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
G9a/GLP complex is associated with Polycomb Repressive Complex 2 (PRC2).The G9a/GLP complex also regulates DNA methylation during early embryogenesis, which is independent of their methyltransferase activity. ZNF644 and WIZ, two zinc finger proteins, interact with G9a and GLP, respectively. These two zinc finger proteins target G9a and GLP to genomic loci for the regulation of gene transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF644 antibody 25956-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |