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验证数据展示

  • WB analysis using 16159-1-AP

    WB analysis using 16159-1-AP

    SK-BR-3 cell lysates were subjected to SDS PAGE followed by western blot with 16159-1-AP (ZMIZ1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 16159-1-AP

    IF Staining of A549 using 16159-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZMIZ1-Specific antibody (16159-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

ZMIZ1-Specific Polyclonal antibody

ZMIZ1-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16159-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ZMIZ1, RAI17, PIAS-like protein Zimp10, PIAS like protein Zimp10, MIZ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSK-BR-3 cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy ZMIZ1 Ready-To-Use IHC Kit. ZMIZ1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16159-1-AP targets ZMIZ1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, MIZ-type containing 1
别名 ZMIZ1, RAI17, PIAS-like protein Zimp10, PIAS like protein Zimp10, MIZ
计算分子量 115 kDa
观测分子量 120 kDa
GenBank蛋白编号NM_020338
基因名称 ZMIZ1
Gene ID (NCBI) 57178
RRIDAB_3669232
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMIZ2 and ZMIZ1 are members of a PIAS-like family of proteins that interact with nuclear hormone receptors. It can act as a transcriptional coactivator, increasing ligand-dependent transcriptional activity of AR and promoting AR sumoylation. The stimulation of AR activity is dependent upon sumolylation. This antibody is specific for ZMIZ1.

实验方案

Product Specific Protocols
WB protocol for ZMIZ1-Specific antibody 16159-1-APDownload protocol
IF protocol for ZMIZ1-Specific antibody 16159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols