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验证数据展示

  • WB analysis using 30640-1-AP (same clone as 30640-1-PBS)

    WB analysis using 30640-1-AP (same clone as 30640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30640-1-AP (ZFP36L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30640-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 30640-1-AP (same clone as 30640-1-PBS)

    IF Staining of mouse heart using 30640-1-AP (same clone as 30640-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ZFP36L2 antibody (30640-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30640-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 30640-1-AP (same clone as 30640-1-PBS)

    IF Staining of HEK-293 using 30640-1-AP (same clone as 30640-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ZFP36L2 antibody (30640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30640-1-PBS in a different storage buffer formulation.

  • WB analysis of HL-60 using 30640-1-AP (same clone as 30640-1-PBS)

    WB analysis of HL-60 using 30640-1-AP (same clone as 30640-1-PBS)

    WB result of ZFP36L2 antibody (30640-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZFP36L2 transfected HL-60 cells. This data was developed using the same antibody clone with 30640-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 30640-1-AP (same clone as 30640-1-PBS)

    IHC staining of mouse kidney using 30640-1-AP (same clone as 30640-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30640-1-AP (ZFP36L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30640-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ZFP36L2 Polyclonal antibody, PBS Only

ZFP36L2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30640-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30640-1-PBS targets ZFP36L2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32428

Product name: Recombinant human ZFP36L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 292-482 aa of BC005010

Sequence: SCSSASSCSSSASSCSSASAASTPSGAPTCCASAAAAAAAALLYGTGGAEDLLAPGAPCAACSSASCANNAFAFGPELSSLITPLAIQTHNFAAVAAAAYYRSQQQQQQQQQQGLAPPAQPPAPPSATLPAGAAAPPSPPFSFQLPRRLSDSPVFDAPPSPPDSLSDRDSYLSGSLSSGSLSGSESPSLDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 36, C3H type-like 2
别名 BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2
计算分子量 51 kDa
观测分子量54 kDa
GenBank蛋白编号BC005010
基因名称 ZFP36L2
Gene ID (NCBI) 678
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47974
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein, belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich element (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).