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验证数据展示

  • WB analysis of U-937 using 12306-1-AP (same clone as 12306-1-PBS)

    WB analysis of U-937 using 12306-1-AP (same clone as 12306-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 12306-1-AP (same clone as 12306-1-PBS)

    WB analysis of HeLa using 12306-1-AP (same clone as 12306-1-PBS)

    WB result of ZFP36L1/2 antibody (12306-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZFP36L1/2 transfected HeLa cells. This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • IHC staining of rat ovary using 12306-1-AP (same clone as 12306-1-PBS)

    IHC staining of rat ovary using 12306-1-AP (same clone as 12306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • IHC staining of rat ovary using 12306-1-AP (same clone as 12306-1-PBS)

    IHC staining of rat ovary using 12306-1-AP (same clone as 12306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12306-1-AP (same clone as 12306-1-PBS)

    IF Staining of HepG2 using 12306-1-AP (same clone as 12306-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L1/2 antibody (12306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12306-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ZFP36L1/2 Polyclonal antibody, PBS Only

ZFP36L1/2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12306-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ZFP36L1, ZFP36 L1, TPA-induced sequence 11b, mRNA decay activator protein ZFP36L1, ERF-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12306-1-PBS targets ZFP36L1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2952

Product name: Recombinant human ZFP36L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC018340

Sequence: MTTTLVSATIFDLSEVLCKGNKMLNYSAPSAGGCLLDRKAVGTPAGGGFPRRHSVTLPSSKFHQNQLLSSLKGEPAPALSSRDSRFRDRSFSEGGERLLPTQKQPGGGQVNSSRYKTELCRPFEENGACKYGDKCQFAHGIHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRALAGARDLSADRPRLQHSFSFAGFPSAAATAAATGLLDSPTSITPPPILSADDLLGSPTLPDGTNNPFAFSSQELASLFAPSMGLPGGGSPTTFLFRPMSESPHMFDSPPSPQDSLSDQEGYLSSSSSSHSGSDSPTLDNSRRLPIFSRLSISDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 36, C3H type-like 1
别名 ZFP36L1, ZFP36 L1, TPA-induced sequence 11b, mRNA decay activator protein ZFP36L1, ERF-1
计算分子量 338 aa, 36 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC018340
基因名称 ZFP36L1
Gene ID (NCBI) 677
RRIDAB_2737443
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07352
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZFP36L1, also named as Butyrate response factor 1 or TPA-induced sequence 11b, is a 338 amino acid protein, which is phosphorylated by RPS6KA1 at Ser-334 upon phorbol 12-myristate 13-acetate (PMA) treatment. ZFP36 encodes tristetraprolin (TTP) the prototype of a small family of RBPs, called the ZFP36 family, that are characterised by highly conserved tandem CCCH zinc-finger RNA-binding domains [PMID: 10751406]. ZFP36 is a RBP that promotes RNA decay and negatively regulates the expression of the myogenic regulatory factor MyoD by binding to the 3'UTR of MyoD mRNA [PMID: 25815583]. Mouse satellite cells from Zfp36-deficient mice express increased amounts of MyoD and display impaired satellite activation, demonstrating a role for ZFP36 in the maintenance of quiescence [PMID: 25815583]. The functions of the ZFP36L1 and ZFP36L2 family members have not been evaluated in skeletal muscle stem cell fate, but have been shown to act redundantly to promote quiescence during lymphocyte development. ZFP36L1 has been implicated in the persistence of the marginal zone B lymphocyte population. ZFP36L1 exists three isoform through blasting on NCBI database and can be phosphorylated, so the range of the molecular weight of ZFP36L1 is about 40-50 kDa. (PMID: 26180518, PMID: 17030620). This antibody recognizes both ZFP36L1 and ZFP36L2.